Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

Transfection is based on nonviral delivery of nucleic acids or proteins into cells. Viral approaches are being used; nevertheless, their translational capacity is nowadays decreasing due to persistent fear of their safety, therefore creating space for the field of nanotechnology. However, nanomedical approaches introducing static nanoparticles for the delivery of biologically active molecules are very likely to be overshadowed by the vast potential of nanorobotics. We hereby present a rapid nonviral transfection of protein into a difficult-to-transfect prostate cancer cell line facilitated by chemically powered rectangular virus-sized (68 nm x 33 nm) nanorobots. The enhanced diffusion of these biocompatible nanorobots is the key to their fast internalization into cells, happening in a matter of minutes and being up to 6-fold more efficient compared to static nanorobots in a nonfueled environment. The Au/Ag plasmonic nature of these nanorobots makes them simply traceable and allows for their detailed subcellular localization. Protein transfection mediated by such nanorobots is an important step forward, challenging the field of nanomedicine and having potential in future translational medical research.

Automated summary

Transfection involves nonviral delivery of nucleic acids or proteins into cells, with nanotechnology surpassing viral methods due to safety concerns. Nanorobots, particularly chemically powered rectangular nanorobots, show enhanced efficiency in protein transfection by fast internalization and subcellular localization, challenging the field of nanomedicine with potential in translational medical research.