期刊
ACS NANO
卷 15, 期 8, 页码 13077-13084出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.1c02311
关键词
droplet microfluidics; digital hybridization assay; hybrid capture; bioassay; human papillomavirus; amplification-free
类别
资金
- National Key R&D Program of China [2018YFA0902600, 2017YFA0205901]
- National Natural Science Foundation of China [21535001, 81730051, 21761142006]
- Chinese Academy of Sciences [QYZDJSSW-SLH039, 121D11KYSB20170026, XDA16020902]
- Shenzhen Bay Laboratory [SZBL2019062801004]
- Guangdong Innovative and Entrepreneurial Research Team Program [2019ZT08Y191]
- Guangzhou Municipal Science and Technology Bureau [202002020085]
- Tencent Foundation through the XPLORER PRIZE
The study developed a droplet-based digital microfluidic hybridization assay for direct nucleic acid detection with attomolar sensitivity, providing a clinically useful method for detecting HPV without amplification.
Detection of nucleic acid without amplification can avoid problems associated with thermal cycling such as labor-intensiveness and aerosol pollution. Here we develop a droplet-based digital microfluidic hybridization assay for nucleic acid detection with attomolar sensitivity. This assay provides a clinically useful sensitivity for detecting human papillomavirus (HPV) without amplification. The sensitivity is accomplished using femtoliter-sized droplet microfluidics for concentrating enzyme-catalyzed fluorescent products into a detectable signal and magnetic beads for accelerating reaction time. Meanwhile, using magnetic beads and droplet microfluidic chips, we can improve the sampling efficiency over conventional methods. We characterized the sensitivity, selectivity, detection range, stability, and accuracy of our assay. Our assay is 50-fold more sensitive than the traditional hybrid capture assay. The assay without amplification avoids problems of complex handling procedures and aerosol pollution. The direct and sensitive detection of nucleic acid using a droplet microfluidic system provides an early disease diagnosis tool.
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