期刊
ACS CHEMICAL BIOLOGY
卷 16, 期 7, 页码 1243-1254出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.1c00322
关键词
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资金
- NIH-NIGMS [R01 GM125814]
- NIH-NIDDK [R01 DK124617]
- UNC Lineberger Comprehensive Cancer Center
- NSF Graduate Research Fellowship program
- NIH [T32 GM008570]
- NSF Major Research Instrumentation award [CHE-1726291]
- Cancer Center Core Support Grant [P30 CA016086]
This study demonstrates the impact of polymyxin B on the production of the genotoxic metabolite colibactin by Escherichia coli, shedding light on the complex relationship between antibiotic use and gut microbiota composition.
The complex reservoir of metabolite-producing bacteria in the gastrointestinal tract contributes tremendously to human health and disease. Bacterial composition, and by extension gut metabolomic composition, is undoubtably influenced by the use of modern antibiotics. Herein, we demonstrate that polymyxin B, a last resort antibiotic, influences the production of the genotoxic metabolite colibactin from adherent-invasive Escherichia coli (AIEC) NC101. Colibactin can promote colorectal cancer through DNA double stranded breaks and interstrand cross-links. While the structure and biosynthesis of colibactin have been elucidated, chemical-induced regulation of its biosynthetic gene cluster and subsequent production of the genotoxin by E. coli are largely unexplored. Using a multiomic approach, we identified that polymyxin B stress enhances the abundance of colibactin biosynthesis proteins (Clb's) in multiple pks+ E. coli strains, including pro-carcinogenic AIEC, NC101; the probiotic strain, Nissle 1917; and the antibiotic testing strain, ATCC 25922. Expression analysis via qPCR revealed that increased transcription of clb genes likely contributes to elevated Clb protein levels in NC101. Enhanced production of Clb's by NC101 under polymyxin stress matched an increased production of the colibactin prodrug motif, a proxy for the mature genotoxic metabolite. Furthermore, E. coli with a heightened tolerance for polymyxin induced greater mammalian DNA damage, assessed by quantification of gamma H2AX staining in cultured intestinal epithelial cells. This study establishes a key link between the polymyxin B stress response and colibactin production in pks+ E. coli. Ultimately, our findings will inform future studies investigating colibactin regulation and the ability of seemingly innocuous commensal microbes to induce host disease.
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