Multifunctional Biosensors Made with Self-Healable Silk Fibroin Imitating Skin

We report on robust silk fibroin (SF) gels fabricated by incorporating cellulose nanocrystals (SF/CNC) as a tough unit and photopolymerization of acrylamide as an elastic segment. The addition of CNC affects the refolding process of SF molecules controlled by nucleation via templating, resulting in a stable mesoscopic structure. The gel shows robust mechanical stability (88.8% of initial stress after 1000 compression cycles) and excellent adhesion to various materials. The connected gel can recover its ionic conductivity within 20 s and be stretched to a maximum strain of 498% after healing for 10 h with an efficiency of 95.2%. This multifunctional gel sensor can sensitively detect different toxic gases and small-scale and large-scale human motions in real-time. Its sensitivity is calculated as GF = 3.84 at 0-200% strain. Especially, the gel with 5 wt % thermochromic pigments as a visual temperature indicator can quickly reflect abnormal human body temperature according to the color change. Therefore, the strategy shows potential applications in flexible electrodes, biomimetic sensors, and visual biosensors.

Automated summary

The study demonstrates that incorporating cellulose nanocrystals with acrylamide photopolymerization can produce robust silk fibroin gels with excellent mechanical stability and adhesion to various materials. The gel can rapidly recover its ionic conductivity within 20 seconds and stretch to a maximum strain of 498% after healing for 10 hours with an efficiency of 95.2%.