期刊
LIFE-BASEL
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/life11050427
关键词
CCD-986Sk; LPS; NTS; MSM; TLR4; NF-κ B; ROS; anti-inflammatory effect
资金
- Nara Bio Co., Ltd., the Republic of Korea
- National Research Foundation of Korea (NRF) - Ministry of Education [2018R1D1A1B07048651, 2019R1I1A1A01060399, 2019R1I1A1A01060537, 2020R1I1A2073517]
- National Research Foundation of Korea [2018R1D1A1B07048651, 2020R1I1A2073517, 2019R1I1A1A01060537, 2019R1I1A1A01060399] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study found that sulfur compounds NTS and MSM can inhibit LPS-induced inflammatory responses by suppressing TLR4 activation and NF-kappa B signaling, reducing nuclear binding of NF-kappa B to proinflammatory cytokines COX-2, IL-1 beta, and IL-6, as well as inhibiting ROS accumulation and DNA damage.
Lipopolysaccharide (LPS)-induced inflammatory response leads to serious damage, up to and including tumorigenesis. Natural mineral sulfur, non-toxic sulfur (NTS), and methylsulfonylmethane (MSM) have anti-inflammatory activity that may inhibit LPS-induced inflammation. We hypothesized that sulfur compounds could inhibit LPS-induced inflammatory responses in CCD-986Sk skin fibroblasts. We used Western blotting and real-time PCR to analyze molecular signaling in treated and untreated cultures. We also used flow cytometry for cell surface receptor analysis, comet assays to evaluate DNA damage, and ELISA-based cytokine detection. LPS induced TLR4 activation and NF-kappa B signaling via canonical and protein kinase C (PKC)-dependent pathways, while NTS and MSM downregulated that response. NTS and MSM also inhibited LPS-induced nuclear accumulation and binding of NF-kappa B to proinflammatory cytokines COX-2, IL-1 beta, and IL-6. Finally, the sulfur compounds suppressed LPS-induced ROS accumulation and DNA damage in CCD-986Sk cells. These results suggest that natural sulfur compounds could be used to treat inflammation and may be useful in the development of cosmetics.
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