期刊
LIFE-BASEL
卷 11, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/life11040301
关键词
cleared cochlea; ribbon synapse; confocal laser scanning microscopy
资金
- German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) [SCHE 1663/3-1]
- Open Access Publication Fund of Hannover Medical School (MHH)
Recent research has found that auditory synaptopathies play a crucial role in sensorineural hearing loss, and cochlear implant procedures may lead to physiological hearing loss in patients with residual hearing. The study developed a new method to examine the inner ear at the synapse level, aiming to better understand the cascade of hearing pathologies with the goal of developing targeted therapeutics.
In recent years sensorineural hearing loss was found to affect not exclusively, nor at first, the sensory cells of the inner ear. The sensory cells' synapses and subsequent neurites are initially damaged. Auditory synaptopathies also play an important role in cochlear implant (CI) care, as they can lead to a loss of physiological hearing in patients with residual hearing. These auditory synaptopathies and in general the cascades of hearing pathologies have been in the focus of research in recent years with the aim to develop more targeted and individually tailored therapeutics. In the current study, a method to examine implanted inner ears of guinea pigs was developed to examine the synapse level. For this purpose, the cochlea is made transparent and scanned with the implant in situ using confocal laser scanning microscopy. Three different preparation methods were compared to enable both an overview image of the cochlea for assessing the CI position and images of the synapses on the same specimen. The best results were achieved by dissection of the bony capsule of the cochlea.
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