期刊
LIFE-BASEL
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/life11030241
关键词
Chloranthus japonicas; PPARγ adipocyte; obesity; metabolism
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2020R1A2B5B02001592, NRF-2020R1I1A1A01074938]
Chloranthus japonicus has been found to promote adipocyte differentiation, with its hexane extract identified as the most potent inducer. Mechanistic studies showed that CJHE acts on the Wnt pathway to stimulate adipogenesis. This natural product may have potential use in treating metabolic syndrome.
Chloranthus japonicus has been heavily investigated for the treatment of various diseases. This paper attempts to show that Chloranthus japonicus can modulate adipocyte differentiation of preadipocytes. To establish this, we investigated the effects of Chloranthus japonicus extract in peroxisome proliferator-activated receptor gamma (PPAR gamma) expression, adipogenesis, and the underlying molecular mechanisms in C3H10T1/2 and 3T3-L1 cells. Our data showed that Chloranthus japonicus methanol extract increased lipid accumulation and promoted adipocyte differentiation. Further studies on the fractionation with various solvents led to the identification of Chloranthus japonicus hexane extract (CJHE) as the most potent inducer of adipocyte differentiation. CJHE consistently increased lipid accumulation and adipocyte marker expression including Ppar gamma and it acted during the early stages of adipocyte differentiation. Mechanistic studies revealed that CJHE and a Wnt inhibitor similarly stimulated adipogenesis and were active in Wnt-selective reporter assays. The effects of CJHE were inhibited by Wnt3a protein treatment and were significantly blunted in beta-catenin-silenced cells, further suggesting that CJHE acted on Wnt pathways to promote adipogenesis. We also showed that Chloranthus japonicus extracts generated from different plant parts similarly promoted adipocyte differentiation. These results identified Chloranthus japonicus as a pro-adipogenic natural product and suggest its potential use in metabolic syndrome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据