期刊
TOXICS
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/toxics9040070
关键词
Caretta caretta; RNA-seq; differential expression; methylmercury; transcriptomics
资金
- Office of Research, Creation, and Innovation of the Universidad Jorge Tadeo Lozano [340-07-10]
- Intramural Research Program of the United States National Institutes of Health (NIH), National Library of Medicine (NLM), National Center for Biotechnology Information (NCBI) [ZIA LM082713-06]
Exposure of loggerhead turtle erythrocytes to MeHg led to changes in gene expression patterns, affecting cellular processes such as cell cycle regulation, lysosomal activity, autophagy, and mitochondrial function. Despite a low response of antioxidant machinery to MeHg, the RBCs maintained constitutive expression of proteins for defense against ROS induced by MeHg.
To understand changes in enzyme activity and gene expression as biomarkers of exposure to methylmercury, we exposed loggerhead turtle erythrocytes (RBCs) to concentrations of 0, 1, and 5 mg L-1 of MeHg and de novo transcriptome were assembled using RNA-seq. The analysis of differentially expressed genes (DEGs) indicated that 79 unique genes were dysregulated (39 upregulated and 44 downregulated genes). The results showed that MeHg altered gene expression patterns as a response to the cellular stress produced, reflected in cell cycle regulation, lysosomal activity, autophagy, calcium regulation, mitochondrial regulation, apoptosis, and regulation of transcription and translation. The analysis of DEGs showed a low response of the antioxidant machinery to MeHg, evidenced by the fact that genes of early response to oxidative stress were not dysregulated. The RBCs maintained a constitutive expression of proteins that represented a good part of the defense against reactive oxygen species (ROS) induced by MeHg.
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