Phytochemicals, Antioxidant Attributes and Larvicidal Activity of Mercurialis annua L. (Euphorbiaceae) Leaf Extracts against Tribolium confusum (Du Val) Larvae (Coleoptera; Tenebrionidae)

Simple Summary Mercurialis annua L. is a Euphorbiacea widespread in the Euro-Mediterranean region. This species is known for its toxicity and is used in traditional pharmacopoeia. However, the chemical composition of the aerial parts of this taxon and their larvicidal impact remain to be explored. However, the literature reports only a few studies on the relationship between the polyphenolic profile of the different extracts of Mercurialis annua L. and the larvicidal activity; therefore, we tested different extracts of M. annua against the larvae of Tribolium confusum (Du Val) for better protection of stored foodstuffs attacked by this pest. On the other hand, green chemistry as a distinct discipline has developed in recent years, which allowed us to identify different active compounds by LC-MS, and study the relationship between larvicidal activity and the chemical composition of extracts from male and female leaves of Mercurialis annua L. This study reports the link between larvicidal activity and the phytochemical composition of male and female leaf extracts of Mercurialis annua L. (M. annua) from four Tunisian regions: Bizerte, Jandouba, Nabeul and Beja. Their antioxidant activity was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assays. Phenolic compounds were identified and quantified using liquid chromatography coupled with a UV detector and mass spectrometry (LC-UV-ESI/MS). Higher antioxidant activity (AOA) was found in the leaves of male plant extracts than of female ones. The leaves of male and female plant extracts from Bizerte exhibited the highest AOA: 22.04 and 22.78 mg Trolox equivalent/g dry matter (mg TE/g DM), respectively. For both sexes, plant extracts from Beja had the lowest AOA with 19.71 and 19.67 mg TE/g DM for male and female plants, respectively. Some phenolic compounds such as narcissin, gallocatechin, rutin, epigallocatechin and epicatechin were identified and quantified using LC-MS, which highlighted the abundance of narcissin and rutin in the male leaves of M. annua. We noted that the interaction between the sex of plants and the provenance had a significant effect on TFC (F = 6.63; p = 0.004) and AOA (F = 6.53; p = 0.004) assays, but there was no interaction between sex and origins for TPC (F = 1.76; p = 0.19). The larvicidal activity of aqueous leaf extracts of M. annua against Tribolium confusum (Du Val) (T. confusum), an insect pest of flour and cereal seeds, showed that the mortality could reach 100% after 48 h of exposure in the Bizerte region. The LC50 values for the leaf extract were low in Bizerte, with 0.003 and 0.009 g/mL for male and female plants, respectively, succeeded by Jandouba, which displayed 0.006 and 0.024 g/mL for male and female plants, respectively. Nabeul showed 0.025 g/mL for male plants and 0.046 g/mL for female plants and Beja showed 0.037 and 0.072 g/mL for male and female plants, respectively. This is the first time that a study has revealed a negative correlation between the antioxidant activity and the larvicidal activity of the leaf extracts of M. annua with the following correlation coefficients of Perason: r = -0.975 and r = -0.760 for male and female plants, respectively.

Automated summary

This study investigated the larvicidal and antioxidant activity of different extracts of Mercurialis annua L. against Tribolium confusum larvae. The results showed that male plant extracts exhibited higher antioxidant activity compared to female ones, and a negative correlation was found between antioxidant activity and larvicidal activity in the leaf extracts of M. annua.