期刊
ISCIENCE
卷 24, 期 6, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2021.102530
关键词
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资金
- Spanish Ministry of Economy and Competitiveness (MINECO) [SAF2017-82886-R]
- Comunidad de Madrid [S2017/BMD-3671-INFLAMUNE-CM]
- Ramon Areces Foundation Ciencias de la Vida y la Salud'' (XIX Concurso-2018)
- Ayudas Fundacion BBVA an Equipos de Investigacion Cientifica (BIOMEDICINA-2018)
- la Caixa'' Banking Foundation [HR17-00016]
- BIOIMID from Instituto de Salud Carlos III [PIE13/041]
- CIBER Cardiovascular (Fondo de Investigacion Sanitaria del Instituto de Salud Carlos III) [CB16/11/00272]
- CIBER Cardiovascular (Fondo Europeo de Desarrollo Regional FEDER) [CB16/11/00272]
- Ministerio de Economia y Competitividad-Spain
- Pro-CNIC Foundation
- FPU program (Spanish Ministry of Education)
T cell activation results in changes in the miRNA repertoire, with some individual miRNAs being specifically upregulated. Using next-generation sequencing, differential expression of multiple miRNAs not previously linked to T cell activation was identified. Upregulated miRNAs showed a higher frequency of 3' adenylation, which may serve a protective function in the adverse environment and could be utilized to improve miRNA stability for T cell-targeted therapy.
T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modification kinetics in human primary CD4+ T cells upon T cell receptor (TCR) or type I interferon stimulation. This analysis identified differential expression of multiple miRNAs not previously linked to T cell activation. Remarkably, upregulated miRNAs showed a higher frequency of 30 adenylation. TCR stimulation was followed by increased expression of RNA modifying enzymes and the RNA degrading enzymes Dis3L2 and Eri1. In the midst of this adverse environment, 30 adenylationmay serve a protective function that could be exploited to improve miRNA stability for T cell-targeted therapy.
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