期刊
ISCIENCE
卷 24, 期 6, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2021.102537
关键词
-
资金
- Victorian State Government OIS program
- National Health & Medical Research Council (NHMRC) of Australia [APP1127336]
- National Heart Foundation of Australia, via the Future Leader Fellowship Scheme [101789, 100067]
- NHMRC [APP1078985, APP1117835]
The study demonstrates the significant role of OIP5-AS1 in cardiac development and disease, with knockout of this gene leading to exacerbated heart failure in female mice. RNA-sequencing results suggest that OIP5-AS1 regulates pathways impacting mitochondrial function.
Long non-coding RNAs ( lncRNAs) have been demonstrated to influence numerous biological processes, being strongly implicated in the maintenance and physiological function of various tissues including the heart. The lncRNA OIP5-AS1 (1700020I14Rik/Cyrano) has been studied in several settings; however its role in cardiac pathologies remains mostly uncharacterized. Using a series of in vitro and ex vivo methods, we demonstrate that OIP5-AS1 is regulated during cardiac development in rodent and human models and in disease settings in mice. Using CRISPR, we engineered a global OIP5-AS1 knockout (KO) mouse and demonstrated that female KO mice develop exacerbated heart failure following cardiac pressure overload (transverse aortic constriction [TAC]) but male mice do not. RNA-sequencing of wild-type and KO hearts suggest that OIP5-AS1 regulates pathways that impact mitochondrial function. Thus, these findings highlight OIP5-AS1 as a gene of interest in sex-specific differences in mitochondrial function and development of heart failure.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据