Empirical Support for the Pattern of Competitive Exclusion between Insect Parasitic Fungi

Fungal entomopathogens are largely facultative parasites and play an important role in controlling the density of insect populations in nature. A few species of these fungi have been used for biocontrol of insect pests. The pattern of the entomopathogen competition for insect individuals is still elusive. Here, we report the empirical competition for hosts or niches between the inter- and intra-species of the entomopathogens Metarhizium robertsii and Beauveria bassiana. It was found that the synergistic effect of coinfection on virulence increase was not evident, and the insects were largely killed and mycosed by M. robertsii independent of its initial co-inoculation dosage and infection order. For example, >90% dead insects were mycosed by M. robertsii even after immersion in a spore suspension with a mixture ratio of 9:1 for B. bassiana versus M. robertsii. The results thus support the pattern of competitive exclusion between insect pathogenic fungi that occurred from outside to inside the insect hosts. Even being inferior to compete for insects, B. bassiana could outcompete M. robertsii during co-culturing in liquid medium. It was also found that the one-sided mycosis of insects occurred during coinfection with different genotypic strains of either fungi. However, parasexual recombination was evident to take place between the compatible strains after coinfection. The data of this study can help explain the phenomena of the exclusive mycosis of insect individuals, but co-occurrence of entomopathogens in the fields, and suggest that the synergistic effect is questionable regarding the mixed use of fungal parasites for insect pest control.

Automated summary

Fungal entomopathogens, mainly facultative parasites, are important in controlling insect populations in nature. Competition between different species such as Metarhizium robertsii and Beauveria bassiana for insect hosts or niches was studied, revealing that co-infection did not significantly increase virulence and M. robertsii was more effective in killing and infecting insects. Additionally, while B. bassiana could outcompete M. robertsii in liquid medium, parasexual recombination between compatible strains occurred after coinfection, raising questions about the efficacy of mixed use of fungal parasites for insect pest control.