期刊
BIOMEDICINES
卷 9, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9050491
关键词
lipids; cerebrospinal fluid; Parkinson's disease; mass-spectrometry; lipidomics
资金
- Spanish Ministry of Science Innovation and Universities [PID2019-110356RB-I00/AEI/10.13039/501100011033]
- Department of Economic and Business Development from Government of Navarra [0011-1411-2020-000028]
The study identified a significant increase in various lipid species in the CSF of PD patients, indicating a dynamic lipid imbalance associated with different stages and durations of the disease. The results suggest that CSF lipidomics could serve as a promising tool for identifying potential lipid markers in PD and distinguishing between PD and other atypical parkinsonisms.
Lipid metabolism is clearly associated to Parkinson's disease (PD). Although lipid homeostasis has been widely studied in multiple animal and cellular models, as well as in blood derived from PD individuals, the cerebrospinal fluid (CSF) lipidomic profile in PD remains largely unexplored. In this study, we characterized the post-mortem CSF lipidomic imbalance between neurologically intact controls (n = 10) and PD subjects (n = 20). The combination of dual extraction with ultra-performance liquid chromatography-electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC-ESI-qToF-MS/MS) allowed for the monitoring of 257 lipid species across all samples. Complementary multivariate and univariate data analysis identified that glycerolipids (mono-, di-, and triacylglycerides), saturated and mono/polyunsaturated fatty acids, primary fatty amides, glycerophospholipids (phosphatidylcholines, phosphatidylethanolamines), sphingolipids (ceramides, sphingomyelins), N-acylethanolamines and sterol lipids (cholesteryl esters, steroids) were significantly increased in the CSF of PD compared to the control group. Interestingly, CSF lipid dyshomeostasis differed depending on neuropathological staging and disease duration. These results, despite the limitation of being obtained in a small population, suggest extensive CSF lipid remodeling in PD, shedding new light on the deployment of CSF lipidomics as a promising tool to identify potential lipid markers as well as discriminatory lipid species between PD and other atypical parkinsonisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据