期刊
BIOMEDICINES
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9040431
关键词
collagen; strontium; gingival fibroblast; bioprinting; osteoporotic
资金
- Ministry of Science and Technology [MOST 108-2314B-002-040, 109-2314-B-039-017, 109-2224-E-080-001]
- China Medical University Hospital [DMR-110-104]
- National Taiwan University Hospital [109-FTN18]
The study utilized a 3D-printed cell-laden collagen/strontium-doped calcium silicate bi-layer scaffold for periodontal regeneration, showing enhanced osteoporotic bone regeneration. The scaffold increased secretion of relevant proteins and demonstrated guided periodontal regeneration, making it a potential candidate for future clinical applications.
Periodontal disease is a chronic disease that can lead to lose teeth and even tooth loss if left untreated. Osteoporosis and periodontal disease share similar characteristics and associated factors. Current regenerative techniques for periodontal diseases are ineffective in restoring complete function and structural integrity of periodontium due to unwanted migration of cells. In this study, we applied the concept of guided tissue regeneration (GTR) and 3D fabricated gingival fibroblast cell-laden collagen/strontium-doped calcium silicate (SrCS) bi-layer scaffold for periodontal regeneration. The results revealed that the bioactive SrCS had a hydroxyapatite formation on its surface after 14 days of immersion and that SrCS could release Sr and Si ions even after 6 months of immersion. In addition, in vitro results showed that the bi-layer scaffold enhanced secretion of FGF-2, BMP-2, and VEGF from human gingival fibroblasts and increased secretion of osteogenic-related proteins ALP, BSP, and OC from WJMSCs. In vivo studies using animal osteoporotic models showed that the 3D-printed cell-laden collagen/SrCS bi-layer scaffold was able to enhance osteoporotic bone regeneration, as seen from the increased Tb.Th and BV/TV ratio and the histological stains. In conclusion, it can be seen that the bi-layer scaffolds enhanced osteogenesis and further showed that guided periodontal regeneration could be achieved using collagen/SrCS scaffolds, thus making it a potential candidate for future clinical applications.
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