期刊
JOURNAL OF INFLAMMATION RESEARCH
卷 14, 期 -, 页码 1845-1858出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/JIR.S307796
关键词
miR-223; gout; animal models; cytokines; monosodium urate
类别
资金
- National Natural Science Foundation of China [81670801, 81800716]
- Sichuan Science and Technology Program [2017JY0037, 2018JY0498]
- Henry Ford Health System Research Grants for Immunology Program [T71016]
- Sichuan Medical Association [S16027]
- Sichuan Provincial Human Resources and Social Security Department [74]
MiR-223 plays a crucial role in regulating inflammatory response in mouse models of gout, and its deficiency exacerbates inflammation. Lower levels of miR-223 in gout patients suggest that upregulation of miR-223 could be a potential therapeutic strategy for alleviating gouty inflammation.
Objective: MicroRNAs were identified as master-switch molecules limiting acute inflammatory response. This study investigated the potential role of microRNA (miR)-223 in the mechanism of gout. Methods: Wild-type (WT) and miR-223 knock-out (KO) mice were used to evaluate the phenotypes of gout models. Inflammatory cytokines were measured in air pouch and peritoneal cavity lavage fluid. In addition to miR-223 level in gout patients, miR-223 and pro-inflammatory genes were examined in bone marrow-derived macrophages (BMDMs) from mice as well as peripheral blood mononuclear cells from healthy controls (HC) treated with monosodium urate (MSU) crystals in vitro. Results: MiR-223 was up-regulated in the early phase in BMDMs from WT mice after MSU challenge and decreased rapidly, and this was not observed in miR-223 KO mice in vitro. In addition, miR-223 was required for macrophages homeostasis. In comparison with WT mice in vivo, miR-223 deficiency exacerbated swelling index of MSU-induced inflammation in foot pad and ankle joint models. MiR-223 deficiency also markedly aggravated inflammatory cells infiltration and cytokines release including interleukin (IL)-1 beta, IL-6 and monocyte chemotactic protein-1 (MCP-1) in the air pouch and peritonitis models. In the in vitro experiments, miR-223 deficiency promoted the inflammatory response by targeting NLR family pyrin domain containing protein 3 (NLRP3). Besides, miR-223 level was downregulated in gout patients and in HC exposed to MSU in vitro. Conclusion: MiR-223 was down-regulated in gout patients and miR-223 deficiency exacerbated inflammatory response in diverse murine models, suggesting that upregulation of miR-223 could be a potential therapeutic strategy for alleviating gouty inflammation.
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