期刊
NPJ REGENERATIVE MEDICINE
卷 6, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41536-021-00123-5
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资金
- Edmonton Orthopaedic Research Committee
- University of Alberta
- Shantou University
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Alberta Innovates and Alberta Advanced Education
- Government of Alberta
This study introduces a promising approach for long-term storage of viable articular cartilage, addressing the shortage of graft tissue for clinical repair of articular cartilage defects.
Preserving viable articular cartilage is a promising approach to address the shortage of graft tissue and enable the clinical repair of articular cartilage defects in articulating joints, such as the knee, ankle, and hip. In this study, we developed two 2-step, dual-temperature, multicryoprotectant loading protocols to cryopreserve particulated articular cartilage (cubes similar to 1 mm(3) in size) using a mathematical approach, and we experimentally measured chondrocyte viability, metabolic activity, cell migration, and matrix productivity after implementing the designed loading protocols, vitrification, and warming. We demonstrated that porcine and human articular cartilage cubes can be successfully vitrified and rewarmed, maintaining high cell viability and excellent cellular function. The vitrified particulated articular cartilage was stored for a period of 6 months with no significant deterioration in chondrocyte viability and functionality. Our approach enables high-quality long-term storage of viable articular cartilage that can alleviate the shortage of grafts for use in clinically repairing articular cartilage defects.
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