期刊
NEUROBIOLOGY OF STRESS
卷 14, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ynstr.2021.100306
关键词
Glucocorticoid receptor; Hippocampus; miRNAs; Neuroplasticity; Dendrites; Dendritic spines; Sex
资金
- Intramural Research Program of the NIEHS, National Institutes of Health
- FONDECYT [1190899]
Sex differences in the brain, particularly in terms of miRNA expression and neuronal morphology, were investigated in this study by generating a neuron-specific GR knockout mouse model. The results showed a sex-biased effect of GR ablation, with differentially expressed miRNAs and changes in neuronal morphology observed mainly in female mice. The study also identified potential transcription factors involved in miRNA regulation and highlighted pathways related to neuronal arborization and spine morphology affected by GR deletion.
Sex differences in the brain have prompted many researchers to investigate the underlying molecular actors, such as the glucocorticoid receptor (GR). This nuclear receptor controls gene expression, including microRNAs (miRNAs), in non-neuronal cells. Here, we investigated sex-biased effects of GR on hippocampal miRNA expression and neuronal morphology by generating a neuron-specific GR knockout mouse (Emx1-Nr3c1(-/-)). The levels of 578 mature miRNAs were assessed using NanoString technology and, in contrast to males, female Emx1-Nr3c1(-/-) mice showed a substantially higher number of differentially expressed miRNAs, confirming a sex-biased effect of GR ablation. Based on bioinformatic analyses we identified several transcription factors potentially involved in miRNA regulation. Functional enrichment analyses of the miRNA-mRNA interactions revealed pathways related to neuronal arborization and both spine morphology and density in both sexes. Two recognized regulators of dendritic morphology, CAMKII-alpha and GSK-3 beta, increased their protein levels by GR ablation in female mice hippocampus, without changes in males. Additionally, sex-specific effects of GR deletion were observed on CA1 neuronal arborization and dendritic spine features. For instance, a reduced density of mushroom spines in apical dendrites was evidenced only in females, while a decreased length in basal dendrites was noted only in males. However, length and arborization of apical dendrites were reduced by GR ablation irrespective of the sex. Overall, our study provides new insights into the sex-biased GR actions, especially in terms of miRNAs expression and neuronal morphology in the hippocampus.
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