期刊
BIOACTIVE MATERIALS
卷 6, 期 4, 页码 1118-1129出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.10.006
关键词
Micro/nanostructured surfaces; Crystalline phase; Hydroxyapatite; Cell response; Bioactivity
资金
- Ministry of Science and Technology of China [2016YFC1100300]
- National Natural Science Foundation of China [21773199, 51571169]
The surface structures and physicochemical properties of titanium implants play a crucial role in osseointegration. Studies have shown that surfaces with micro- and nanoscale roughness can efficiently regulate cell-material interaction. The introduction of ultrathin hydroxyapatite on micro/nanostructured surfaces with optimized crystalline phase benefits cell proliferation, differentiation, and maturation, indicating potential for enhanced implant osseointegration in vivo.
Surface structures and physicochemical properties critically influence osseointegration of titanium (Ti) implants. Previous studies have shown that the surface with both micro- and nanoscale roughness may provide multiple features comparable to cell dimensions and thus efficiently regulate cell-material interaction. However, less attention has been made to further optimize the physicochemical properties (e.g., crystalline phase) and to further improve the bioactivity of micro/nanostructured surfaces. Herein, micro/nanostructured titania surfaces with different crystalline phases (amorphous, anatase and anatase/rutile) were prepared and hydroxyapatite (HA) nanorods were deposited onto the as-prepared surfaces by a spin-assisted layer-by-layer assembly method without greatly altering the initial multi-scale morphology and wettability. The effects of crystalline phase, chemical composition and wettability on osteoblast response were investigated. It is noted that all the micro/nanostructured surfaces with/without HA modification presented superamphiphilic. The activities of MC3T3-E1 cells suggested that the proliferation trend on the micro/nanostructured surfaces was greatly influenced by different crystalline phases, and the highest proliferation rate was obtained on the anatase/rutile surface, followed by the anatase; but the cell differentiation and extracellular matrix mineralization were almost the same among them. After ultrathin HA modification on the micro/nanostructured surfaces with different crystalline phases, it exhibited similar proliferation trend as the original surfaces; however, the cell differentiation and extracellular matrix mineralization were significantly improved. The results indicate that the introduction of ultrathin HA to the micro/nanostructured surfaces with optimized crystalline phase benefits cell proliferation, differentiation and maturation, which suggests a favorable biomimetic microenvironment and provides the potential for enhanced implant osseointegration in vivo.
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