期刊
ACS OMEGA
卷 6, 期 12, 页码 8672-8679出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.1c00606
关键词
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资金
- Natural Science Foundation of China [51903062]
- Guangdong Basic and Applied Basic Research Foundation [2020A1515011320]
- Natural Science Foundation of Guangdong Province of China [2018A030313588]
- Open Laboratory Project of Guangzhou Medical University [B185004159]
- Science and Technology Planning Project of Guangdong Province of China [2016B030309002]
This study developed a pH-responsive nano-prodrug by conjugating emodin to mPEG-PEI with acid-sensitive boronate ester bonds, showing promising potential for pH-responsive tumor therapy and antibacterial activity. The nano-prodrug exhibited a superior cytotoxic effect against HeLa cells compared to free emodin, and effectively inhibited the growth of both Gram-positive and Gram-negative bacteria.
In this study, a pH-responsive nano-prodrug was fabricated by conjugating emodin to the PEGylated polyethyleneimine (mPEG-PEI) with acid-sensitive boronate ester bonds. H-1 NMR spectra results showed that emodin was effectively bonded to mPEG-PEI, and acid- sensitive assay further confirmed the formation of boronate ester bonds. The size and morphology of the nano-prodrug were ascertained through transmission electron microscopy (TEM) and dynamic light scattering (DLS), which showed that the prodrug has a sphere-like shape with hydrodynamic size around 102 nm at pH 7.4. Subsequently, a drug-release behavior assay was carried out to carefully investigate the acid-sensitive drug-delivery property of the prodrug. Moreover, in vitro cell viability assay confirmed the superior cytotoxic effect of the nano-prodrug against HeLa cells compared to free emodin. Furthermore, the antibacterial study showed that the nano-prodrug could inhibit the bacterial (both Gram-positive and Gram-negative) growth more effectively than free emodin. Overall, this study provides a promising paradigm of the multifunctional nano-prodrug for pH-responsive tumor therapy and antibacterial activity.
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