期刊
PLANTS-BASEL
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/plants10050980
关键词
Sphagneticola trilobata; antileukemia; chronic myeloid leukemia (CML); BCR/ABL
资金
- Thu Dau Mot University [DT.20.2-047]
The study found that the methanol extract of Sphagneticola trilobata (L.) Pruski (MeST) has an inhibitory effect on the growth of leukemia cells, especially those harboring imatinib-resistant BCR/ABL. The cell death induced by MeST is through apoptosis and it can also suppress the expression of the BCR/ABL protein.
We will study the effects of the methanol extract of Sphagneticola trilobata (L.) Pruski (Asteraceae) (MeST) on the growth of leukemia cells that may contain the BCR/ABL gene. This study also clarifies the mechanism of this effect on these cells. For this purpose, the cells harboring wildtype BCR/ABL, imatinib-resistant BCR/ABL (K562 and TCCYT315I), or Ba/F3 cells transfected with wild-type or mutant BCR/ABL genes were used. The results showed that MeST effectively inhibited the viability of leukemia cells in both a dose- and time-dependent manner. The effect of MeST seems to be more sensitive in the cells that carry imatinib-resistant BCR/ABL (especially the T315I BCR/ABL mutation) than those with wild-type BCR/ABL. Furthermore, we have demonstrated that the death caused by MeST is apoptosis and the treatment with MeST could suppress the expression of BCR/ABL, subsequently altering the downstream cascade of BCR/ABL such as AKT and MAPK signaling. In conclusion, MeST has been able to suppress the growth of leukemia cells harboring BCR/ABL. The mechanism of the anti-leukemic effect of MeST on cells harboring imatinib-resistant BCR/ABL mutations could be due to the disruption of the BCR/ABL oncoprotein signaling cascade.
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