期刊
FRONTIERS IN ECOLOGY AND EVOLUTION
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fevo.2021.664835
关键词
genome-wide identification; sequence variations; SSR marker development; munake grape; cultivar
类别
资金
- Basic Scientific Research Funding Project of Autonomous Region Public Welfare Scientific Research Institutes (Development and analysis of SNP markers in Munake grape and its progeny based on whole-genome resquencing)
- Forestry Reform and Development from the Central Government [Xin(2021) TG04]
- China Agriculture Research System [CARS-29-ZP-08]
- Tianshan YouthExcellence Youth Project funds project [2018Q037]
The study utilized whole-genome sequencing to reveal the genetic information of the Munake grape cultivar, including millions of mutation markers and thousands of tags. The research validated some of the markers and delved deeper into the genetic makeup of the Munake cultivar.
The Munake grape cultivar produces uniquely flavored and high-quality fruits. Despite the numerous beneficial agronomic traits of Munake, there are few genetic resources available for this cultivar. To address this knowledge gap, the entire genome was sequenced using whole-genome sequencing approaches and compared with a Vitis vinifera L. reference genome. This study describes more than 3 million single nucleotide polymorphism (SNP), 300,000 insertion and deletion (InDel), 14,000 structural variation (SV), and 80,000 simple sequence repeat (SSR) markers (one SSR per 4.23 kb), as well as their primers. Among the SSRs, 44 SSR primer pairs were randomly selected and validated by polymerase chain reaction (PCR), allowing discrimination between the different Munake cultivar genotypes. The genetic data provided allow a deeper understanding of Munake cultivar genomic sequence and contribute to better knowledge of the genetic basis behind its key agronomic traits.
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