期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2021.676065
关键词
surface-enhanced raman scattering (SERS); enzymatic amplification; circulating tumor DNA (ctDNA); DNA nanotechnology; biomarker
资金
- National Key Research and Development Program of China [2017YFE0131700]
- National Natural Science Foundation of China [21874096, 21575095]
This study presents a new method integrating cycled enzymatic DNA amplification and Au nanoparticle@silicon-assisted surface-enhanced Raman scattering (SERS) technique for sensitive detection of single-base-mutated ctDNA sequences in diluted blood samples. The approach can be applied in translational medicine for early cancer diagnosis, therapeutic effect monitoring, and prognosis of patients.
Circulating tumor DNA (ctDNA) represents an emerging biomarker of liquid biopsies for the development of precision cancer diagnostics and therapeutics. However, sensitive detection of ctDNA remains challenging, due to their short half-life and low concentrations in blood samples. In this study, we report a new method to address this challenge by integrating cycled enzymatic DNA amplification technique and Au nanoparticle@silicon-assisted surface-enhanced Raman scattering (SERS) technique. We have demonstrated a reproducible identification of a single-base-mutated ctDNA sequence of diffuse intrinsic pontine gliomas (DIPGs), with the limit of detection (LOD) as low as 9.1 fM in the spiked blood samples. This approach can be used to analyze trace amounts of ctDNA in translational medicine for early diagnosis, therapeutic effect monitoring, and prognosis of patients with cancer.
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