期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2021.655662
关键词
cell adhesion; receptor-ligand binding; nanoscale lipid cluster; ligand mobility; phase separation; binding constant
资金
- NSFC Basic Science Center Program for Multiscale Problems in Nonlinear Mechanics [11988102]
- Youth Innovation Promotion Association CAS
- National Natural Science Foundation of China [11902327, 11972041]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB22040102]
The study found that the binding of mobile ligands to adhesion receptors can promote the formation of mesoscale lipid domains, while in the case of immobile ligands, the binding and coalescence of nanoscale lipid clusters depend on the distribution of ligands on the substrate.
Cell-cell adhesion and the adhesion of cells to extracellular matrix are mediated by the specific binding of receptors on the cell membrane to their cognate ligands on the opposing surface. The adhesion receptors can exhibit affinity for nanoscale lipid clusters that form in the cell membrane. Experimental studies of such adhesion systems often involve a cell adhering either to a solid surface with immobile ligands or a supported lipid bilayer with mobile ligands. A central question in these cell-substrate adhesions is how the mobility of the ligands physically affects their binding to the adhesion receptors and thereby the behavior of the nanoscale lipid clusters associated with the receptors. Using a statistical mechanical model and Monte Carlo simulations for the adhesion of cells to substrates with ligands, we find that, for mobile ligands, binding to adhesion receptors can promote the formation of mesoscale lipid domains, which in turn enhances the receptor-ligand binding. However, in the case of immobile ligands, the receptor-ligand binding and the tendency for the nanoscale lipid clusters to further coalesce depend on the distribution of the ligands on the substrate. Our findings help to explain why different adhesion experiments for identifying the interplay between receptor-ligand binding and heterogeneities in cell membranes led to contradictory results.
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