期刊
MICROORGANISMS
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9040770
关键词
S; Typhi porins; vaccination; circulating memory; T cells
类别
资金
- Instituto Mexicano del Seguro Social (IMSS) [FIS/IMSS/PROT/MD/1690, FIS/IMSS/PROT/EMER/1890, FIS/IMSS/PROT/IT19/1881]
- Mexican National Science and Technology Council (CONACYT) [FORDECYT/33SE/2020/10/22-03]
- CONACYT
- National Autonomous University of Mexico (UNAM)
- IMSS
Vaccination with Salmonella enterica serovar Typhi porins induces skin T cell responses, with IFN-gamma- and IL-17-producing cells, and contributes to protection against S. Typhi. The study highlights the importance of circulating memory T cell responses in the optimal protection provided by porins, showcasing a mechanism that could aid in vaccine development.
Salmonella enterica serovar Typhi (S. Typhi) porins, OmpC and OmpF, are potent inducers of the immune response against S. Typhi in mice and humans. Vaccination with porins induces the protection against 500 LD50 of S. Typhi, life-lasting bactericidal antibodies and effector T cell responses in mice; however, the nature of the memory T cell compartment and its contribution to protection remains unknown. In this work, we firstly observed that vaccination with porins induces in situ (skin) CD4+ and CD8+ T cell responses. Analysis of the porin-specific functional responses of skin CD4+ and CD8+ T cells showed IFN-gamma- and IL-17-producing cells in both T cell populations. The memory phenotype of porin-specific T cells indicated the presence of resident and effector memory phenotypes in the skin, and a central memory phenotype in the skin-draining lymph node. In addition, we demonstrated that vaccination with porins via skin reduces the bacterial burden following challenge. Finally, evaluating the role of the circulating T cell memory population in protection, we showed that circulating memory CD4+ and CD8+ T cells are crucial in porin-mediated protection against S. Typhi. Overall, this study highlights the importance of inducing circulating memory T cell responses in order to achieve the optimal protection provided by porins, showing a mechanism that could be sought in the rational development of vaccines.
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