期刊
MICROORGANISMS
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9040765
关键词
capripox; lumpy skin disease; goatpox; sheeppox; LSDV; GTPV; SPPV; species-specific; TaqMan; MGB
类别
Outbreaks of three capripox virus species have significant impacts on animal health and economies, classified as notifiable diseases by the OIE. A study developed sensitive duplex qPCR assays using fluorescence-labelled probes for rapid, reliable, and robust detection, differentiation, and characterization of capripox virus isolates.
Outbreaks of the three capripox virus species, namely lumpy skin disease virus, sheeppox virus, and goatpox virus, severely affect animal health and both national and international economies. Therefore, the World Organization for Animal Health (OIE) classified them as notifiable diseases. Until now, discrimination of capripox virus species was possible by using different conventional PCR protocols. However, more sophisticated probe-based real-time qPCR systems addressing this issue are, to our knowledge, still missing. In the present study, we developed several duplex qPCR assays consisting of different types of fluorescence-labelled probes that are highly sensitive and show a high analytical specificity. Finally, our assays were combined with already published diagnostic methods to a diagnostic workflow that enables time-saving, reliable, and robust detection, differentiation, and characterization of capripox virus isolates.
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