期刊
ANTIOXIDANTS
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/antiox10050704
关键词
raspberry; metabolomics; high resolution mass spectrometry; data independent acquisition; phenolic compounds; pomological characterization
资金
- Regione Toscana [PIF 40/2015]
This study provided a detailed analysis of antioxidant and bioactive compounds in different varieties of Rubus idaeus L. fruits, compared to Rubus occidentalis L. black raspberry. Certain unique compounds were identified for the first time in raspberry fruits, and differences in metabolic traits were observed between different raspberry varieties.
This study provided a detailed profiling of the antioxidant and bioactive compounds occurring in three varieties of Rubus idaeus L. fruits (Fall Gold, Glen Ample and Tulameen) compared to Rubus occidentalis L. black raspberry (Jewel cultivar), adopting a comprehensive untargeted metabolomics approach developed with UHPLC analysis coupled with quadrupole/time-of-flight high resolution mass spectrometry, using the SWATH(R) acquisition protocol. The feature selection and annotation workflow, applied to the analysis of raspberry extracts in both polarities, allowed identifying 68 bioactive compounds mainly belonging to the classes of (poly)phenolic compounds. Interestingly, some of these identifications (e.g., ferulic acid glycosides and the ellagitannin-like nobotanin/malabathrin) represent the first report in raspberry fruits. Principal component analysis made possible highlighting the features more related to the expression of a genotype effect within the R. idaeus species or between the two raspberry species herein investigated. Overall, flavanols were the most discriminating features for the Fall Gold variety, whereas ellagitannins and flavonol glycosides represent more distinctive metabolic traits in Glen Ample and Tulameen fruits. Moreover, R. occidentalis Jewel variety was strongly characterized by the occurrence of anthocyanins, such as cyanidin, pelargonidin and delphinidin glycosides.
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