期刊
BIOMOLECULES
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/biom11050753
关键词
povidone-iodine; SARS-CoV-2; inflammation; antiviral; Zika; Chikungunya
资金
- NIH [R01EY032149, 1R21AI140033]
- Research to Prevent Blindness
- NIH center grant [P30EY004068]
PVP-I demonstrates potent antiviral effects against ZIKV and CHIKV infection of ocular cells in vitro, primarily by direct viral inactivation to attenuate the virus' ability to infect host cells, without causing cellular toxicity.
Several RNA viruses, including SARS-CoV-2, can infect or use the eye as an entry portal to cause ocular or systemic diseases. Povidone-Iodine (PVP-I) is routinely used during ocular surgeries and eye banking as a cost-effective disinfectant due to its broad-spectrum antimicrobial activity, including against viruses. However, whether PVP-I can exert antiviral activities in virus-infected cells remains elusive. In this study, using Zika (ZIKV) and Chikungunya (CHIKV) virus infection of human corneal and retinal pigment epithelial cells, we report antiviral mechanisms of PVP-I. Our data showed that PVP-I, even at the lowest concentration (0.01%), drastically reduced viral replication in corneal and retinal cells without causing cellular toxicity. Antiviral effects of PVP-I against ZIKV and CHIKV were mediated by direct viral inactivation, thus attenuating the ability of the virus to infect host cells. Moreover, one-minute PVP-I exposure of infected ocular cells drastically reduced viral replication and the production of infectious progeny virions. Furthermore, viral-induced (CHIKV) expression of inflammatory genes (TNF-alpha, IL-6, IL-8, and IL1 beta) were markedly reduced in PVP-I treated corneal epithelial cells. Together, our results demonstrate potent antiviral effects of PVP-I against ZIKV and CHIKV infection of ocular cells. Thus, a low dose of PVP-I can be used during tissue harvesting for corneal transplants to prevent potential transmission of RNA viruses via infected cells.
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