Xanthurenic Acid Is the Main Pigment of Trichonephila clavata Gold Dragline Silk

Spider silk is a natural fiber with remarkable strength, toughness, and elasticity that is attracting attention as a biomaterial of the future. Golden orb-weaving spiders (Trichonephila clavata) construct large, strong webs using golden threads. To characterize the pigment of golden T. clavata dragline silk, we used liquid chromatography and mass spectrometric analysis. We found that the major pigment in the golden dragline silk of T. clavata was xanthurenic acid. To investigate the possible function of the pigment, we tested the effect of xanthurenic acid on bacterial growth using gram-negative Escherichia coli and gram-positive Bacillus subtilis. We found that xanthurenic acid had a slight antibacterial effect. Furthermore, to investigate the UV tolerance of the T. clavata threads bleached of their golden color, we conducted tensile deformation tests and scanning electron microscope observations. However, in these experiments, no significant effect was observed. We therefore speculate that golden orb-weaving spiders use the pigment for other purposes, such as to attract their prey in the sunlight.

Automated summary

Spider silk, known for its strength, toughness, and elasticity, has attracted attention as a potential biomaterial. Through analysis, it was found that the pigment in the golden dragline silk of golden orb-weaving spiders is xanthurenic acid, which has a slight antibacterial effect. Despite bleaching the golden color of the threads, no significant change in UV tolerance was observed, leading to speculation that the spiders use the pigment for other purposes.