期刊
VACCINES
卷 9, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/vaccines9050470
关键词
virus-like particles (VLPs); chimeric VLPs; nanoparticles; vaccine platform; RHDV; FMDV; B-cell epitope; T-cell epitope; immune response; pig
资金
- Spanish Ministry of Science and Innovation [AGL201676445-R, PID2019-107145RB-I00, BFU2017-88736]
- Comunidad de Madrid
- ECFEDER funds [P2018/BAA-4370 PLATESA 2, P2018/NMT-4389]
- Spanish Ministry of Science and Innovation (FPI programme)
Chimeric RHDV VLPs displaying FMDV-derived epitopes elicit a robust neutralizing immune response in mice and pigs, affording partial clinical protection against an FMDV challenge in pigs.
Currently there is a clear trend towards the establishment of virus-like particles (VLPs) as a powerful tool for vaccine development. VLPs are tunable nanoparticles that can be engineered to be used as platforms for multimeric display of foreign antigens. We have previously reported that VLPs derived from rabbit hemorrhagic disease virus (RHDV) constitute an excellent vaccine vector, capable of inducing specific protective immune responses against inserted heterologous T-cytotoxic and B-cell epitopes. Here, we evaluate the ability of chimeric RHDV VLPs to elicit immune response and protection against Foot-and-Mouth disease virus (FMDV), one of the most devastating livestock diseases. For this purpose, we generated a set of chimeric VLPs containing two FMDV-derived epitopes: a neutralizing B-cell epitope (VP1 (140-158)) and a T-cell epitope [3A (21-35)]. The epitopes were inserted joined or individually at two different locations within the RHDV capsid protein. The immunogenicity and protection potential of the chimeric VLPs were analyzed in the mouse and pig models. Herein we show that the RHDV engineered VLPs displaying FMDV-derived epitopes elicit a robust neutralizing immune response in mice and pigs, affording partial clinical protection against an FMDV challenge in pigs.
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