期刊
VACCINES
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/vaccines9040363
关键词
heat stability; Newcastle disease virus; paramyxovirus; vaccine quality; vaccine stability
资金
- Bill andMelinda Gates Foundation [OPP1176784]
- UK aid from the UK Government through GALVmed [300504]
- Bill and Melinda Gates Foundation [OPP1176784] Funding Source: Bill and Melinda Gates Foundation
The commercial I-2 strains showed superior thermal stability at 37 degrees Celsius compared to other ND vaccines, but all vaccines dissolved at 51 and 61 degrees Celsius within 2 to 4 hours. This study highlights the importance of vaccine formulation-specific factors and cold chain maintenance for vaccine efficacy in hot climates.
Vaccination against Newcastle disease (ND), a devastating viral disease of chickens, is often hampered by thermal inactivation of the live vaccines, in particular in tropical and hot climate conditions. In the past, thermostable vaccine strains (I-2) were proposed to overcome this problem but previous comparative studies did not include formulation-specific factors of commercial vaccines. In the current study, we aimed to verify the superior thermal stability of commercially formulated I-2 strains by comparing six commercially available ND vaccines. Subjected to 37 degrees C as lyophilized preparations, two vaccines containing I-2 strains were more sensitive to inactivation than a third I-2 vaccine or compared to three other vaccines based on different ND strains. However, reconstitution strains proved to have a comparable tenacity. Interestingly, all vaccines still retained a sufficient virus dose for protection (10(6) EID50) after 1 day at 37 degrees C. These results suggest that there are specific factors that influence thermal stability beyond the strain-specific characteristics. Exposing ND vaccines to elevated temperatures of 51 and 61 degrees C demonstrated that inactivation of all dissolved vaccines including I-2 vaccine strains occurred within 2 to 4 h. The results revealed important differences among the vaccines and emphasize the importance of the quality of a certain vaccine preparation rather than the strain it contains. These data highlight that regardless of the ND strain used for vaccine preparation, the appropriate cold chain is mandatory for keeping live ND vaccines efficiency in hot climates.
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