期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.653800
关键词
laccase; Trametes versicolor; emodin; proteomics analysis; CLSM
资金
- Project of Anhui University Innovation and Entrepreneurship Training Program [201810357291, 201810357045, 201510357364]
- China Postdoctoral Science Foundation [4139ZRL]
Secondary metabolites of traditional Chinese herbs can effectively induce laccase production in white rot fungi, with emodin identified as a potent inducer. Emodin may affect the expression of laccase genes through three mechanisms: reducing bioenergy productivity, the AHR/XRE pathway, and the Nrf2/ARE pathway. Additionally, emodin could reduce ATP synthesis by lowering mitochondrial membrane potential, leading to subsequent responses.
Secondary metabolites of traditional Chinese herbs can prominently stimulate the production of laccase from white rot fungi during submerged fermentation. However, the molecular mechanism through which these natural products induce the production of laccase remains unknown. In this study, the Chinese herbal medicine Polygonum cuspidatum was used to induce laccase production in Trametes versicolor, and the best inducer was identified in emodin, even under conditions of 1000-L, large-scale fermentation. Proteomics analysis identified a selection of proteins that were differentially expressed in the presence of emodin, indicating that emodin may affect the expression of laccase genes through three mechanisms: reducing bioenergy productivity, the aryl hydrocarbon receptor (AHR)/xenobiotic response element (XRE) pathway, and the nuclear erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway. Combined with protoplast flow cytometry and fluorescence, it is revealed that emodin might reduce the synthesis of ATP by lowering the mitochondrial membrane potential, leading to the subsequent responses.
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