期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.638382
关键词
cartilage; chondrocyte; osteoarthritis; necroptosis; receptor-interacting protein kinase 1; extracellular matrix
资金
- National Natural Science Foundation of China [32000923, 81972101, 81871770]
- Beijing Municipal Natural Science Foundation [7214304, 7171014]
The study shows that RIP1 plays a crucial role in the onset and progression of osteoarthritis (OA) by mediating chondrocyte necroptosis and disrupting extracellular matrix (ECM) metabolism. Inhibition of RIP1 activity protects rats from cartilage degradation and limb pain, and bone morphogenetic protein 7 (BMP7) is identified as a downstream target that mediates RIP1-induced chondrocyte necroptosis and OA manifestations. This provides a new therapeutic target for OA.
Osteoarthritis (OA) is a highly prevalent and debilitating joint disorder that characterized by progressive destruction of articular cartilage. There is no effective disease-modifying therapy for the condition due to limited understanding of the molecular mechanisms on cartilage maintenance and destruction. Receptor-interacting protein kinase 1 (RIP1)-mediated necroptosis plays a vital role in various diseases, but the involvement of RIP1 in OA pathogenesis remains largely unknown. Here we show that typical necrotic cell morphology is observed within human OA cartilage samples in situ, and that RIP1 is significantly upregulated in cartilage from both OA patients and experimental OA rat models. Intra-articular RIP1 overexpression is sufficient to induce structural and functional defects of cartilage in rats, highlighting the crucial role of RIP1 during OA onset and progression by mediating chondrocyte necroptosis and disrupting extracellular matrix (ECM) metabolism homeostasis. Inhibition of RIP1 activity by its inhibitor necrostatin-1 protects the rats from trauma-induced cartilage degradation as well as limb pain. More importantly, we identify bone morphogenetic protein 7 (BMP7) as a novel downstream target that mediates RIP1-induced chondrocyte necroptosis and OA manifestations, thereby representing a non-canonical regulation mode of necroptosis. Our study supports a model whereby the activation of RIP1-BMP7 functional axis promotes chondrocyte necroptosis and subsequent OA pathogenesis, thus providing a new therapeutic target for OA.
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