4.7 Article

Natural mucosal barriers and COVID-19 in children

期刊

JCI INSIGHT
卷 6, 期 9, 页码 -

出版社

AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/jci.insight.148694

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资金

  1. NIH [R01 AI134367, UL1 TR002556, P30 AI124414]
  2. Albert Einstein College of Medicine Dean's COVID-19 Pilot Research Award
  3. NIGMS MSTP training grant [T32GM007288, T32 AI007501]
  4. Eric J. Heyer, MD, PhD Translational Research Pilot Project Award

向作者/读者索取更多资源

Children infected with SARS-CoV-2 show a more robust early mucosal immune response compared to adults, which may contribute to favorable clinical outcomes. Higher levels of IFN signaling and other innate pathways were observed in children, along with increased levels of certain cytokines in nasal fluid, suggesting a more protective immune response in children against severe COVID-19.
BACKGROUND. Coronavirus disease 2019 (COVID-19) is more benign in children compared with adults for unknown reasons. This contrasts with other respiratory viruses where disease manifestations are often more severe in children. We hypothesize that a more robust early innate immune response to SARS coronavirus 2 (SARS-CoV-2) protects against severe disease. METHODS. Clinical outcomes, SARS-CoV-2 viral copies, and cellular gene expression were compared in nasopharyngeal swabs obtained at the time of presentation to the emergency department from 12 children and 27 adults using bulk RNA sequencing and quantitative reverse-transcription PCR. Total protein, cytokines, and anti-SARS-CoV-2 IgG and IgA were quantified in nasal fluid. RESULTS. SARS-CoV-2 copies, angiotensin-converting enzyme 2, and TMPRSS2 gene expression were similar in children and adults, but children displayed higher expression of genes associated with IFN signaling, NLRP3 inflammasome, and other innate pathways. Higher levels of IFN-alpha 2, IFN-gamma, IP-10, IL-8, and IL-1 beta protein were detected in nasal fluid in children versus adults. Children also expressed higher levels of genes associated with immune cells, whereas expression of those associated with epithelial cells did not differ in children versus adults. Anti-SARS-CoV-2 IgA and IgG were detected at similar levels in nasal fluid from both groups. None of the children required supplemental oxygen, whereas 7 adults did (P = 0.03); 4 adults died. CONCLUSION. These findings provide direct evidence of a more vigorous early mucosal immune response in children compared with adults and suggest that this contributes to favorable clinical outcomes.

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