期刊
NATURE BIOMEDICAL ENGINEERING
卷 5, 期 7, 页码 713-725出版社
NATURE RESEARCH
DOI: 10.1038/s41551-021-00706-z
关键词
-
资金
- NSF [2048283]
- Open Philanthropy award
- NSF INTERN award [1827671]
- Cardea
- Directorate For Engineering
- Div Of Industrial Innovation & Partnersh [1827671] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [2048283] Funding Source: National Science Foundation
Liquid-gated graphene field-effect transistors can distinguish single-point mutations in human genomic samples, opening up new possibilities for genetic research and diagnostics.
Liquid-gated graphene field-effect transistors anchoring guide RNA-Cas9 complexes can be used to discriminate between single-point mutations in human genomic samples. Simple and fast methods for the detection of target genes with single-nucleotide specificity could open up genetic research and diagnostics beyond laboratory settings. We recently reported a biosensor for the electronic detection of unamplified target genes using liquid-gated graphene field-effect transistors employing an RNA-guided catalytically deactivated CRISPR-associated protein 9 (Cas9) anchored to a graphene monolayer. Here, using unamplified genomic samples from patients and by measuring multiple types of electrical response, we show that the biosensors can discriminate within one hour between wild-type and homozygous mutant alleles differing by a single nucleotide. We also show that biosensors using a guide RNA-Cas9 orthologue complex targeting genes within the protospacer-adjacent motif discriminated between homozygous and heterozygous DNA samples from patients with sickle cell disease, and that the biosensors can also be used to rapidly screen for guide RNA-Cas9 complexes that maximize gene-targeting efficiency.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据