期刊
AGRONOMY-BASEL
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/agronomy11050845
关键词
epigenetic; potato; somatic fusion; MSAP; HPLC
资金
- National Science Centre in Poland [UMO2012/07/B/NZ9/01901]
- Plant Breeding and Acclimatization Institute-National Research Institute from Polish Ministry of Education and Science
The research revealed that somatic hybridization in potato leads to an increase in DNA methylation levels, affecting the epigenetics of the hybrids. Variations were observed in the DNA methylation levels between wild potato species and cultivated varieties.
Interspecific somatic hybridization is a noteworthy breeding strategy that allows the production of novel genetic variability when crossing barriers exist between two parental species. Although the genetic consequences of somatic hybridization have been well documented, little is known on its impact at the epigenetic level. The objective of our research was to investigate the epigenetic changes, in particular DNA methylation, occurring in a population of potato somatic hybrids. The analysis of 96 Solanum x michoacanum (+) S. tuberosum somatic hybrids from five fusion combinations and their parents was carried out by methylation-sensitive amplified polymorphism (MSAP) and high-performance liquid chromatography (HPLC) methods. Six MSAP primer combinations generated 622 unique bands, of which 295 were fully methylated. HPLC analysis showed from 15.5% to 16.9% total cytosine methylation within the parental forms. Overall, the MSAP and HPLC methods indicated an increase in DNA methylation in the somatic hybrids in comparison to their parents. Among the latter, a lower degree of DNA methylation in the wild S. x michoacanum species than S. tuberosum was found. Our findings indicated that somatic hybridization changed the level of cytosine methylation in the studied potato somatic hybrids.
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