期刊
AGRONOMY-BASEL
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/agronomy11051001
关键词
flavonoid 3 ',5 '-hydroxylase; Glycine soja; pinkish-white flower; soybean; W1 locus
资金
- Kyungpook National University Research Fund
In this study, a new color variant of wild soybean with pinkish-white flowers was isolated, and it was found that the pinkish-white color was caused by a single recessive allele of W1. A SNP detected in the mutant led to an amino acid substitution in a highly conserved domain of F3'5'H proteins, resulting in hypofunctional F3'5'H activity and the pinkish-white color of the flowers.
The enzyme flavonoid 3',5'-hydroxylase (F3'5'H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3'5'H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the flower's pinkish-white color is caused by w1-s3, a single recessive allele of W1. The SNP detected in the mutant caused amino acid substitution (A(304)S) in a highly conserved SRS4 domain of F3'5'H proteins. On the basis of the results of the protein variation effect analyzer (PROVEAN) tool, we suggest that this mutation may lead to hypofunctional F3'5'H activity rather than non-functional activity, which thereby results in its pinkish-white color.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据