期刊
VIROLOGICA SINICA
卷 36, 期 5, 页码 968-980出版社
KEAI PUBLISHING LTD
DOI: 10.1007/s12250-021-00353-3
关键词
Autographa californica multiple nucleopolyhedrovirus (AcMNPV); orf13; Nucleocapsid egress; OB morphogenesis
类别
资金
- National Key Research and Development Program of China [2017YFD0201206]
- WIV One-Three-Five'' strategic program [Y602111SA1]
The orf13 gene of AcMNPV plays a crucial role in the efficient nuclear egress of nucleocapsids during virion budding, while being dispensable for occlusion body formation. The encoded protein colocalizes with the nuclear lamina and deletion of ac13 significantly reduces virion budding from infected cells.
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) orf13 (ac13) is a conserved gene in all sequenced alphabaculoviruses. However, its function in the viral life cycle remains unknown. In this study, we found that ac13 was a late gene and that the encoded protein, bearing a putative nuclear localization signal motif, colocalized with the nuclear lamina. Deletion of ac13 did not affect viral genome replication, nucleocapsid assembly or occlusion body (OB) formation, but reduced virion budding from infected cells by approximately 400-fold compared with the wild-type virus. Deletion of ac13 substantially impaired the egress of nucleocapsids from the nucleus to the cytoplasm, while the OB morphogenesis was unaffected. Taken together, our results indicated that ac13 was required for efficient nuclear egress of nucleocapsids during virion budding, but was dispensable for OB formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据