期刊
FRONTIERS IN ONCOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2021.663360
关键词
B-cell acute lymphocytic leukemia; CDK7 inhibitor; cell apoptosis; metabolism; c-MYC
类别
资金
- National Key R&D Program of China, Stem Cell and Translation Research [2016YFA0102000]
- National Natural Science Foundation of China [81870082, 82070158, 81800191]
- Guangxi Natural Science Foundation Program [2019GXNSFDA245031]
- Dawn Program of Shanghai Education Commission [19SG14]
- Innovation Program of Shanghai Municipal Education Commission [YG2017MS31]
- Shanghai Science and Technology Committee [18411968100]
THZ1 inhibits cell cycle and induces apoptosis of B-ALL cells, disrupts cellular metabolic pathways, suppresses cellular metabolism by downregulating c-MYC-mediated metabolic enzymes, and enhances cell apoptosis through upregulation of p53 expression, providing a novel treatment option for B-ALL.
B-cell acute lymphocytic leukemia (B-ALL) is a malignant blood cancer that develops in children and adults and leads to high mortality. THZ1, a covalent cyclin-dependent kinase 7 (CDK7) inhibitor, shows anti-tumor effects in various cancers by inhibiting cell proliferation and inducing apoptosis. However, whether THZ1 has an inhibitory effect on B-ALL cells and the underlying mechanism remains obscure. In this study, we showed that THZ1 arrested the cell cycle of B-ALL cells in vitro in a low concentration, while inducing the apoptosis of B-ALL cells in vitro in a high concentration by activating the apoptotic pathways. In addition, RNA-SEQ results revealed that THZ1 disrupted the cellular metabolic pathways of B-ALL cells. Moreover, THZ1 suppressed the cellular metabolism and blocked the production of cellular metabolic intermediates in B-ALL cells. Mechanistically, THZ1 inhibited the cellular metabolism of B-ALL by downregulating the expression of c-MYC-mediated metabolic enzymes. However, THZ1 treatment enhanced cell apoptosis in over-expressed c-MYC B-ALL cells, which was involved in the upregulation of p53 expression. Collectively, our data demonstrated that CDK7 inhibitor THZ1 induced the apoptosis of B-ALL cells by perturbing c-MYC-mediated cellular metabolism, thereby providing a novel treatment option for B-ALL.
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