期刊
CELLS
卷 10, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/cells10040763
关键词
renovascular disease; microvasculature; revascularization; mesenchymal stem; stromal cells; extracellular vesicles
类别
资金
- NIH [DK122137, DK106427, DK120292, DK122734]
This study showed that mesenchymal stem cell-derived extracellular vesicles (EVs) offer superior protection of the stenotic kidney microvasculature and greater attenuation of renal injury and fibrosis compared to percutaneous transluminal renal angioplasty (PTRA), despite both strategies similarly improving renal hemodynamics and function. The EVs were found to be enriched with pro-angiogenic, anti-inflammatory, and antioxidants genes, highlighting their potential as a promising therapeutic intervention in renovascular disease.
Background: Percutaneous transluminal renal angioplasty (PTRA) confers clinical and mortality benefits in select 'high-risk' patients with renovascular disease (RVD). Intra-renal-delivered extracellular vesicles (EVs) released from mesenchymal stem/stromal cells (MSCs) protect the kidney in experimental RVD, but have not been compared side-by-side to clinically applied interventions, such as PTRA. We hypothesized that MSC-derived EVs can comparably protect the post-stenotic kidney via direct tissue effects. Methods: Five groups of pigs (n = 6 each) were studied after 16 weeks of RVD, RVD treated 4 weeks earlier with either PTRA or MSC-derived EVs, and normal controls. Single-kidney renal blood flow (RBF) and glomerular filtration rate (GFR) were assessed in vivo with multi-detector CT, and renal microvascular architecture (3D micro CT) and injury pathways ex vivo. Results: Despite sustained hypertension, EVs conferred greater improvement of intra-renal microvascular and peritubular capillary density compared to PTRA, associated with attenuation of renal inflammation, oxidative stress, and tubulo-interstitial fibrosis. Nevertheless, stenotic kidney RBF and GFR similarly rose in both PTRA- and EV-treated pigs compared RVD + Sham. mRNA sequencing reveled that EVs were enriched with pro-angiogenic, anti-inflammatory, and antioxidants genes. Conclusion: MSC-derived EVs elicit a better preservation of the stenotic kidney microvasculature and greater attenuation of renal injury and fibrosis compared to PTRA, possibly partly attributed to their cargo of vasculo-protective genes. Yet, both strategies similarly improve renal hemodynamics and function. These observations shed light on diverse mechanisms implicated in improvement of post-stenotic kidney function and position EVs as a promising therapeutic intervention in RVD.
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