期刊
CELLS
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/cells10051032
关键词
mesenchymal stem cells; characterisation; epigenetic; methylation; hydroxymethylation; physiological oxygen; DNA methyltransferase
类别
资金
- Iraqi Ministry of Higher Education and Scientific Research, University of Baghdad, Baghdad, Iraq
Research has shown that physoxia affects the DNA methylation patterns and enzymatic activity in human mesenchymal stem cells, resulting in upregulation of SOX2, decreased levels of 5mC and 5hmC, and reduced transcription of DNA methyltransferase and Ten-eleven translocation enzyme. This suggests that oxygen sensitivity plays a role in epigenetic regulation within stem cells, potentially influencing their therapeutic development.
The application of physiological oxygen (physoxia) concentrations is becoming increasingly commonplace within a mammalian stem cell culture. Human mesenchymal stem cells (hMSCs) attract widespread interest for clinical application due to their unique immunomodulatory, multi-lineage potential, and regenerative capacities. Descriptions of the impact of physoxia on global DNA methylation patterns in hMSCs and the activity of enzymatic machinery responsible for its regulation remain limited. Human bone marrow-derived mesenchymal stem cells (BM-hMSCs, passage 1) isolated in reduced oxygen conditions displayed an upregulation of SOX2 in reduced oxygen conditions vs. air oxygen (21% O-2,O- AO), while no change was noted for either OCT-4 or NANOG. DNA methylation marks 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) showed decreases in 2% O-2 environment (workstation) (2% WKS). DNMT3B (DNA methyltransferase 3B) and TET1 (Ten-eleven translocation enzyme 1) displayed reduced transcription in physoxia. Consistent with transcriptional downregulation, we noted increased promoter methylation levels of DNMT3B in 2% WKS accompanied by reduced DNMT3B and TET1 protein expression. Finally, a decrease in HIF1A (Hypoxia-inducible factor 1A) gene expression in 2% WKS environment correlated with protein levels, while HIF2A was significantly higher in physoxia correlated with protein expression levels vs. AO. Together, these data have demonstrated, for the first time, that global 5mC, 5hmC, and DNMT3B are oxygen-sensitive in hMSCs. Further insights into the appropriate epigenetic regulation within hMSCs may enable increased safety and efficacy development within the therapeutic ambitions.
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