期刊
CELLS
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/cells10030640
关键词
cadmium; DNA damage; chromatin structure; epigenetic modifications; gamma-H2AX; oxidative stress; replication stress; Vicia faba
类别
资金
- National Science Centre [2019/03/X/NZ3/00477]
The toxicity of cadmium to plant cells is mainly mediated by the generation of ROS and DNA damage-induced replication stress, ultimately activating signaling factors involved in cell cycle control pathways, DNA repair systems, and epigenetic adaptations.
Among heavy metals, cadmium is considered one of the most toxic and dangerous environmental factors, contributing to stress by disturbing the delicate balance between production and scavenging of reactive oxygen species (ROS). To explore possible relationships and linkages between Cd(II)-induced oxidative stress and the consequent damage at the genomic level (followed by DNA replication stress), root apical meristem (RAM) cells in broad bean (V. faba) seedlings exposed to CdCl2 treatment and to post-cadmium recovery water incubations were tested with respect to H2O2 production, DNA double-strand breaks (gamma-phosphorylation of H2AX histones), chromatin morphology, histone H3S10 phosphorylation on serine (a marker of chromatin condensation), mitotic activity, and EdU staining (to quantify cells typical of different stages of nuclear DNA replication). In order to evaluate Cd(II)-mediated epigenetic changes involved in transcription and in the assembly of nucleosomes during the S-phase of the cell cycle, the acetylation of histone H3 on lysine 5 (H3K56Ac) was investigated by immunofluorescence. Cellular responses to cadmium (II) toxicity seem to be composed of a series of interlinked biochemical reactions, which, via generation of ROS and DNA damage-induced replication stress, ultimately activate signal factors engaged in cell cycle control pathways, DNA repair systems, and epigenetic adaptations.
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