期刊
CELLS
卷 10, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/cells10040917
关键词
flow cytometry; kidney neoplasms; lymphocytes; tumor-infiltrating; tumor microenvironments
类别
资金
- National Research Foundation of Korea - Ministry of Education, Republic of Korea [NRF-2017R1A2B4011780]
- Ministry of Science and ICT [NRF-2017R1A2B4010568]
The study presents the immune microenvironment pattern of MESTK to oncogenic stress, revealing the distribution and characteristics of TILs in the tumor stroma. High levels of effector memory T cells were found in MESTK samples, along with other activated B cell populations.
Mixed epithelial and stromal tumor of the kidney (MESTK), a benign rare tumor with malignant transformation potential, is thought to be derived from fetal or immature cells originating from the mesonephric and Mullerian ducts. However, due to its rarity, little is known about the anti-tumor immune responses in MESTK. Herein, we present five cases of MESTK and evaluate the population of tumor-infiltrating lymphocytes (TILs) using a freshly obtained MESTK sample. Microscopically, TILs were scattered or clustered in large aggregates in the stroma in all five cases; furthermore, three cases exhibited heavy, large lymphocytic aggregates with no well-organized tertiary lymphoid structures with germinal centers. Flow cytometric analysis of TILs in one freshly obtained MESTK sample revealed that >40% of CD3(+) T cells were effector memory Fas(+)CD28(-) gamma delta T cells expressing high levels of programmed cell death protein 1 and inducible T-cell co-stimulator, but low levels of CD44 and CD27. Most alpha ss T cells exhibited a naive phenotype. Additionally, we detected many activated class-switched CD21(+)CD27(+) B cells as well as CD11c(high)IgM(high) marginal zone B-like and CD27(-)CD21(-)CD23(-) immunoglobulin (Ig)D(high)IgM(low) age-associated B-like cells. Collectively, for the first time, we report the immune microenvironment pattern of MESTK to oncogenic stress.
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