期刊
CELLS
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/cells10051013
关键词
endometrium; primary cell culture; kynurenine; indoleamine 2; 3-dioxygenase 1; RAGE
类别
资金
- National Centre for Research and Development [II/269364/5/NCBR/2015]
- European Union from European Regional Development Fund under the Operational Programme Development of Eastern Poland [POPW.01.03.0006-003/09-00]
In this study, primary human endometrial cells derived from cancerous and noncancerous tissues were cultured in vitro to study the expression of immune modulatory factors RAGE and IDO1. It was found that cancer endometrial cell culture exhibited increased Kyn secretion compared to the control group, suggesting a potential association between IDO1 activity and cancer phenotype.
Background: Immune modulatory factors like indoleamine 2,3-dioxygenase 1 (IDO1) generating kynurenine (Kyn) and receptor for advanced glycation end-products (RAGE) contribute to endometrial and cancer microenvironment. Using adequate experimental models is needed to learn about the significance of these molecular factors in endometrial biology. In this paper we study IDO1 activity and RAGE expression in the in vitro cultured primary human endometrial cells derived from cancerous and noncancerous tissue. Methods: The generated primary cell cultures from cancer and noncancerous endometrial tissues were characterized using immunofluorescence and Western Blot for expression of endometrial and cancer markers. IDO1 activity was studied by Kyn quantification with High Performance Liquid Chromatography with Diode Array Detector. Results: The primary cultures of endometrial cells were obtained with 80% success rate and no major genetic aberrations. The cells retained in vitro expression of markers (mucin MUC1 and HER2) or immunomodulatory factors (RAGE and IDO1). Increased Kyn secretion was associated with cancer endometrial cell culture in contrast to the control one. Conclusions: Primary endometrial cells express immune modulatory factors RAGE and IDO1 in vitro associated with cancer phenotype of endometrium.
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