期刊
JOURNAL OF CLINICAL MEDICINE
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/jcm10091976
关键词
Interleukin-31; histamine-4-receptor; atopic dermatitis
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [2020R1C1C1011296]
- National Research Foundation of Korea [2020R1C1C1011296] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study revealed that CD45RO(+)CLA(+)H4R(+) T cells are an important source of IL-31 in AD patients. Stimulation with 4-methylhistamine increased IL-31 production in these cells. This suggests that these cells could be a potential target for treating IL-31-induced pruritus.
IL-31 is involved in pruritus in atopic dermatitis (AD) and the pathogenesis of AD. However, the mechanism of IL-31 production is not fully understood. We sought to investigate the association between CD45RO(+)CLA(+)H4R(+) T cells and IL-31 production. Immunofluorescence studies were performed retrospectively on punch-biopsy specimens from five people with AD and three healthy controls. In addition, blood samples were collected prospectively from eight patients with AD and eight healthy controls for sorting CD45RO(+)CLA(+)H4R(+) T cells. There was no overlap of patients between the biopsy group and the blood sampling group. Sorted cells were stimulated with 4-methylhistamine (4MH), and the level of IL-31 was measured by an enzyme-linked immunosorbent assay. Immunofluorescence showed co-localization of H4R and IL-31 in lesional AD skin but not in normal skin of healthy controls. The proportion of CLA(+)H4R(+) T cells among CD3(+)CD45RO(+) lymphocytes was 18.3 +/- 6.2% in patients with AD and 11.2 +/- 7.6% in healthy controls. In the AD group, production of IL-31 by CD45RO(+)CLA(+)H4R(+) T cells increased from 32.4 +/- 13.3 pg/mL to 47.5 +/- 18.7 pg/mL by 4MH stimulation after 24 h (p < 0.001). However, in the control group, production of IL-31 was 20.1 +/- 10.6 pg/mL without and 22.1 +/- 9.3 pg/mL with 4MH stimulation (p > 0.05). According to our study, CD45RO(+)CLA(+)H4R(+) T cells are an important source of IL-31 in AD, and may be a target for treatment of IL-31-induced pruritus.
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