期刊
SCIENCE ADVANCES
卷 7, 期 12, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abe5940
关键词
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资金
- National University of Singapore (NUS)
- NUS Research Scholarship
- National Medical Research Council
- Ministry of Education
- Institute for Health Innovation Technology
- IMCB Independent Fellowship
- NUS Early Career Research Award
- National University Health System Seed Grant
- National Centre for Infectious Diseases Grant
- National Medical Research Council [MOH-000234]
CATCH is a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in clinical samples, achieving superior performance and versatile implementation.
Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in native clinical samples. The technology, termed catalytic amplification by transition-state molecular switch (CATCH), leverages DNA-enzyme hybrid complexes to form a molecular switch. By ratiometric tuning of its constituents, the multicomponent molecular switch is prepared in a hyperresponsive state-the transition state-that can be readily activated upon the binding of sparse RNA targets to turn on substantial enzymatic activity. CATCH thus achieves superior performance (similar to 8 RNA copies/mu l), direct fluorescence detection that bypasses all steps of PCR (<1 hour at room temperature), and versatile implementation (high-throughput 96-well format and portable microfluidic assay). When applied for clinical COVID-19 diagnostics, CATCH demonstrated direct and accurate detection in minimally processed patient swab samples.
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