4.8 Article

DEAD-box helicases modulate dicing body formation in Arabidopsis

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SCIENCE ADVANCES
卷 7, 期 18, 页码 -

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AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abc6266

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资金

  1. National Natural Science Foundation of China [NSFC 32090012, 91954105, 31622046, 31900224, 31872302]
  2. Ministry of Agriculture transgenic major projects [2016ZX08010001]
  3. National Key Plan for Scientific Research and Development of China [2017YFD0200904, 2019YFC1200504]
  4. Beijing Municipal Natural Science Foundation [5202017]
  5. Program of CAS [ZDBS-LY-SM027]
  6. Strategic Priority Research Program of the CAS [XDB11050700]

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The RNA helicases RH6, RH8, and RH12 play key roles in modulating the formation of membraneless nuclear dicing bodies (D-bodies) in Arabidopsis via liquid-liquid phase separations (LLPSs). Their accumulation decreases in the nuclei upon Turnip mosaic virus infections, leading to a decrease in D-bodies.
Eukaryotic cells contain numerous membraneless organelles that are made from liquid droplets of proteins and nucleic acids and that provide spatiotemporal control of various cellular processes. However, the molecular mechanisms underlying the formation and rapid stress-induced alterations of these organelles are relatively uncharacterized. Here, we investigated the roles of DEAD-box helicases in the formation and alteration of membraneless nuclear dicing bodies (D-bodies) in Arabidopsis thaliana. We uncovered that RNA helicase 6 (RH6), RH8, and RH12 are previously unidentified D-body components. These helicases interact with and promote the phase separation of SERRATE, a key component of D-bodies, and drive the formation of D-bodies through liquid-liquid phase separations (LLPSs). The accumulation of these helicases in the nuclei decreases upon Turnip mosaic virus infections, which couples with the decrease of D-bodies. Our results thus reveal the key roles of RH6, RH8, and RH12 in modulating D-body formation via LLPSs.

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