期刊
ACS SENSORS
卷 6, 期 5, 页码 1933-1939出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.1c00329
关键词
programmable sensors; structure-guided design; protein engineering; synthetic biology; BTEX; luciferase
资金
- DBT (Govt. of India) [BT/INF/22/SP23026/2017]
- Wadhwani Research Center for Bioengineering [WRCB DGDON446]
- DBT [BT/PR18927/BCE/8/1376/2016]
- IIT Bombay
By utilizing synthetic biology approaches and structure-guided design, a series of whole-cell biosensors were created, with one sensor capable of detecting harmful substances in drinking water at a sensitivity of 1 ppb. By engineering mutations into the gene, a variety of sensors for detecting different pollutants were generated.
Aromatics such as phenols, benzene, and toluene are carcinogenic xenobiotics which are known to pollute water resources. By employing synthetic biology approaches combined with a structure-guided design, we created a tunable array of whole-cell biosensors (WCBs). The MopR genetic system that has the natural ability to sense and degrade phenol was adapted to detect phenol down to similar to 1 ppb, making this sensor capable of directly detecting phenol in permissible limits in drinking water. Importantly, by using a single WCB design, we engineered mutations into the MopR gene that enabled generation of a battery of sensors for a wide array of pollutants. The engineered WCBs were able to sense inert compounds like benzene and xylene which lack active functional groups, without any loss in sensitivity. Overall, this universal programmable biosensor platform can be used to create WCBs that can be deployed on field for rapid testing and screening of suitable drinking water sources.
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