期刊
FOOD BIOSCIENCE
卷 42, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fbio.2021.101045
关键词
Anti-Helicobacter pylori probiotic; Principal component analysis; 16S ribosomal RNA gene Sequence
资金
- Liaoning Province Natural Science Foundation of China [2020-MZLH-39]
- Educational Commission of Liaoning Province of China [J2019017]
- Program of University Innovation Team in Liaoning Province
- National Key R&D Program of China [2018YFC1604205]
This study isolated and identified two potential probiotic Lactobacillus strains from healthy infant feces, which showed inhibitory effects on Helicobacter pylori. These strains significantly reduced the viable count of H. pylori when co-cultured with live cells or cell-free supernatants.
Helicobacter pylori is considered as the causative agent of gastrointestinal diseases. The large increase of drug-resistant strains caused by the treatment of H. pylori makes it very important to develop new treatment strategies. This study aimed to isolate and select Lactobacillus strains from infant feces and evaluate the inhibitory effects against H. pylori. Six strains were initially screened out of Forty-seven Lactobacillus strains obtained from healthy infants feces through evaluating acid (pH 3.0) and bile salt tolerance (0.3%). Based on the re-screening of the four indexes of transit tolerance, hydrophobicity, self-aggregation and anti-Helicobacter pylori, combined with the comprehensive evaluation of principal component analysis, these both strains of IF-3083 and IF-5014 were obtained. And these two strains were identified as Lactobacillus plantarum using 16S ribosomal RNA gene sequences. Also, the two identified strains showed good adhesion to human gastric adenocarcinoma (AGS) and human colorectal adenocarcinoma (HT-29) cells, gamma-hemolytic activity and susceptibility to seven antibiotics. The viable count of H. pylori SS1 was significantly decreased when co-cultured with the live cells (LC) or cell-free supernatants (CFS) of these two strains. These preliminary results suggested that L. plantarum IF-3083 and L. plantarum IF-5014 had the potential to be used as probiotics to prevent H. pylori infection.
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