期刊
INFECTION AND DRUG RESISTANCE
卷 14, 期 -, 页码 1155-1168出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IDR.S303992
关键词
Staphylococcus aureus; MSSA; MRSA; biofilm; antibiotic resistance; crystal violet; Congo red agar; phenotypic assay
资金
- University of Szeged Open Access fund [5175]
- Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/00144/20/5]
- New National Excellence Programme of the Ministry of Human Resources [UNKP-20-5-SZTE-330]
- Ministry of Human Capacities, Hungary [20391-3/2018/FEKUSTRAT]
- ESCMID's 30 under 30 Award
The study investigated the biofilm production rates in MSSA and MRSA isolates from Hungarian hospitals, finding no association between resistance and biofilm formation, while erythromycin, clindamycin, and rifampin resistance were associated with biofilm positivity.
Introduction: Staphylococcus aureus (S. aureus) is an important causative pathogen in human infections. The production of biofilms by bacteria is an important factor, leading to treatment failures. There has been significant interest in assessing the possible relationship between the multidrug-resistant (MDR) status and the biofilm-producer phenotype in bacteria. The aim of our present study was to assess the biofilm-production rates in clinical methicillin-susceptible S. aureus [MSSA] and methicillin-resistant S. aureus [MRSA] isolates from Hungarian hospitals and the correlation between resistance characteristics and their biofilm-forming capacity. Methods: A total of three hundred (n=300) S. aureus isolates (corresponding to MSSA and MRSA isolates in equal measure) were included in this study. Identification of the isolates was carried out using the VITEK 2 ID/AST system and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method and E-tests, confirmation of MRSA status was carried out using PBP2a agglutination assay. Biofilm-production was assessed using the crystal violet (CV) tube-adherence method and the Congo red agar (CRA) plate method. Results: There were significant differences among MSSA and MRSA isolates regarding susceptibility-levels to commonly used antibiotics (in case of erythromycin, clindamycin and ciprofloxacin: p<0.001, gentamicin: p=0.023, sulfamethoxazole/trimethoprim: p=0.027, rifampin: p=0.037). In the CV tube adherence-assay, 37% (n=56) of MSSA and 39% (n=58) of MRSA isolates were positive for biofilm-production, while during the use of CRA plates, 41% (n=61) of MSSA and 44% (n=66) of MRSA were positive; no associations were found between methicillin-resistance and biofilm-production. On the other hand, erythromycin, clindamycin and rifampin resistance was associated with biofilm-positivity (p=0.004, p<0.001 and p<0.001, respectively). Biofilm-positive isolates were most common from catheter-associated infections. Discussion: Our study emphasizes the need for additional experiments to assess the role biofilms have in the pathogenesis of implant-associated and chronic S. aureus infections.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据