期刊
FRONTIERS IN GENETICS
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.673286
关键词
nanocarriers; CRISPR; Cas9; delivery; exosome; modification
资金
- National Natural Science Foundation of China [81772394, 81972116]
- Guangdong International Cooperation Project [2021A0505030011]
- Key Program of Natural Science Foundation of Guangdong Province [2018B0303110003]
- Shenzhen Science and Technology Projects [JCYJ20170817172023838, JCYJ20170306092215436, JCYJ20170413161649437]
- Samming Project of Medicine in Shenzhen [SZSM201612079]
- Key Realm R&D Program of Guangdong Province [2019B030335001]
- Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties [SZGSP013, SZGSP007]
- Shenzhen Key Medical Discipline Construction Fund [SZXK042, SZXK049]
- China Postdoctoral Science Foundation [2020M682907]
Nanocarriers show great potential for improving the delivery efficiency of the CRISPR/Cas system. This review highlights the components of the CRISPR/Cas9 system, the development of nano-systems for delivery, and the emphasis on the exosome-based CRISPR/Cas9 delivery system.
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery.
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